AsLOV2

Photoreceptor AsLOV2
Binding partner /
Cofactor FMN
Source organism Avena sativa
Mode of action intramolecular conformational change
Excitation wavelength 450 nm
Reversion wavelength dark
Excitation time seconds
Reversion time tens of seconds
Crystal structure of AsLOV2, ground state. (PDB ID: 2V1A)

AsLOV2-derived switches:

Related original publications

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  1. Optogenetic dissection of transcriptional repression in a multicellular organism. Nat Commun, 2024
  2. Optogenetically engineered Septin-7 enhances immune cell infiltration of tumor spheroids. Proc Natl Acad Sci U S A, 2024
  3. Cryo-ET of actin cytoskeleton and membrane structure in lamellipodia formation using optogenetics. bioRxiv, 2024
  4. Optogenetic Control of the Mitochondrial Protein Import in Mammalian Cells. Cells, 2024
  5. Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping. Sci Rep, 2024
  6. Optogenetic tools for inducing organelle membrane rupture. bioRxiv, 2024
  7. Optogenetic inhibition of light-captured alcohol-taking striatal engrams facilitates extinction and suppresses reinstatement. Alcohol Clin Exp Res (Hoboken), 2024
  8. Notch1 Phase Separation Coupled Percolation facilitates target gene expression and enhancer looping. bioRxiv, 2024
  9. Multisite Assembly of Gateway Induced Clones (MAGIC): a flexible cloning toolbox with diverse applications in vertebrate model systems. bioRxiv, 2024
  10. OptoLacI: optogenetically engineered lactose operon repressor LacI responsive to light instead of IPTG. Nucleic Acids Res, 2024
  11. Optogenetic inhibition of light-captured alcohol-taking striatal engrams facilitates extinction and suppresses reinstatement. bioRxiv, 2024
  12. Reversible Photoregulation of Cell-Cell Adhesions With Opto-E-cadherin. Bio Protoc, 2024
  13. Kinetic properties of optogenetic DNA editing by LiCre-loxP. bioRxiv, 2024
  14. Ultralow Background Membrane Editors for Spatiotemporal Control of Phosphatidic Acid Metabolism and Signaling ACS Cent Sci, 2024
  15. Quantitative comparison of nuclear transport inhibition by SARS coronavirus ORF6 reveals the importance of oligomerization. Proc Natl Acad Sci U S A, 2024
  16. Engineered poly(A)-surrogates for translational regulation and therapeutic biocomputation in mammalian cells. Cell Res, 2024
  17. Light inducible protein degradation in E. coli with the LOVdeg tag. Elife, 2024
  18. Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. Nat Commun, 2023
  19. Rapid characterization of anti-CRISPR proteins and optogenetically engineered variants using a versatile plasmid interference system. Nucleic Acids Res, 2023
  20. Optogenetic control of YAP reveals a dynamic communication code for stem cell fate and proliferation. Nat Commun, 2023
  21. Optogenetics for sensors: On-demand fluorescent labeling of histone epigenetics. Biochem Biophys Res Commun, 2023
  22. Turn-On Protein Switches for Controlling Actin Binding in Cells. bioRxiv, 2023
  23. Reversible photoregulation of cell-cell adhesions with opto-E-cadherin. Nat Commun, 2023
  24. Photoactivatable base editors for spatiotemporally controlled genome editing in vivo. Biomaterials, 2023
  25. Cell Cycle Control by Optogenetically Regulated Cell Cycle Inhibitor Protein p21. Biology (Basel), 2023
  26. Optogenetic control of Cdc48 for dynamic metabolic engineering in yeast. Metab Eng, 2023
  27. Light-switchable transcription factors obtained by direct screening in mammalian cells. Nat Commun, 2023
  28. Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nat Methods, 2023
  29. OptIC Notch reveals mechanism that regulates receptor interactions with CSL. Development, 2023
  30. Optogenetic control of YAP can enhance the rate of wound healing. Cell Mol Biol Lett, 2023
  31. Engineering of NEMO as calcium indicators with large dynamics and high sensitivity. Nat Methods, 2023
  32. Requirements for mammalian promoters to decode transcription factor dynamics. Nucleic Acids Res, 2023
  33. Allosteric inactivation of an engineered optogenetic GTPase. Proc Natl Acad Sci U S A, 2023
  34. Light inducible protein degradation in E. coli with LOVtag. bioRxiv, 2023
  35. An engineered N-acyltransferase-LOV2 domain fusion protein enables light-inducible allosteric control of enzymatic activity. J Biol Chem, 2023
  36. Engineering of bidirectional, cyanobacteriochrome-based light-inducible dimers (BICYCL)s. Nat Methods, 2023
  37. LILAC: enhanced actin imaging with an optogenetic Lifeact. Nat Methods, 2023
  38. Two Rac1 pools integrate the direction and coordination of collective cell migration. Nat Commun, 2022
  39. Analysis of Slow-Cycling Variants of the Light-Inducible Nuclear Protein Export System LEXY in Mammalian Cells. ACS Synth Biol, 2022
  40. Spatial and temporal control of expression with light-gated LOV-LexA. G3 (Bethesda), 2022
  41. Optogenetic control of RelA reveals effect of transcription factor dynamics on downstream gene expression. bioRxiv, 2022
  42. A genetically encoded photo-proximity labeling approach for mapping protein territories. bioRxiv, 2022
  43. Optogenetic control of YAP cellular localisation and function. EMBO Rep, 2022
  44. Optical control of protein delivery and partitioning in the nucleolus. Nucleic Acids Res, 2022
  45. Local temporal Rac1-GTP nadirs and peaks restrict cell protrusions and retractions. Sci Adv, 2022
  46. Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation. Cell Rep, 2022
  47. MYC amplifies gene expression through global changes in transcription factor dynamics. Cell Rep, 2022
  48. Quantification of nuclear transport inhibition by SARS-CoV-2 ORF6 using a broadly applicable live-cell dose-response pipeline. bioRxiv, 2021
  49. Analysis of Three Architectures for Controlling PTP1B with Light. ACS Synth Biol, 2021
  50. Two-input protein logic gate for computation in living cells. Nat Commun, 2021
  51. Desensitisation of Notch signalling through dynamic adaptation in the nucleus. EMBO J, 2021
  52. Mechanical worrying drives cell migration in crowded environments. bioRxiv, 2021
  53. Extremely rapid and reversible optogenetic perturbation of nuclear proteins in living embryos. Dev Cell, 2021
  54. Mechanosensitivity of nucleocytoplasmic transport. bioRxiv, 2021
  55. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
  56. Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells. J Vis Exp, 2021
  57. O-GlcNAc modification of nuclear pore complexes accelerates bidirectional transport. J Cell Biol, 2021
  58. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
  59. Rab10-Positive Tubular Structures Represent a Novel Endocytic Pathway That Diverges From Canonical Macropinocytosis in RAW264 Macrophages. Front Immunol, 2021
  60. A photo-switchable yeast isocitrate dehydrogenase to control metabolic flux through the citric acid cycle. bioRxiv, 2021
  61. Light-dependent N-end rule-mediated disruption of protein function in Saccharomyces cerevisiae and Drosophila melanogaster. PLoS Genet, 2021
  62. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
  63. Rac1 activation can generate untemplated, lamellar membrane ruffles. BMC Biol, 2021
  64. Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein. Nucleic Acids Res, 2021
  65. Optogenetic control of calcium influx in mammalian cells. Methods Enzymol, 2021
  66. Structural Determinants for Light-Dependent Membrane Binding of a Photoswitchable Polybasic Domain. ACS Synth Biol, 2021
  67. A single-chain and fast-responding light-inducible Cre recombinase as a novel optogenetic switch. Elife, 2021
  68. Blue Light‐Operated CRISPR/Cas13b‐Mediated mRNA Knockdown (Lockdown). Adv Biol, 2021
  69. Optogenetic Control of Myocardin‐Related Transcription Factor A Subcellular Localization and Transcriptional Activity Steers Membrane Blebbing and Invasive Cancer Cell Motility. Adv Biol, 2021
  70. Design of Smart Antibody Mimetics with Photosensitive Switches. Adv Biol (Weinh), 2021
  71. High levels of Dorsal transcription factor downregulate, not promote, snail expression by regulating enhancer action. bioRxiv, 2021
  72. Engineering of a bona fide light-operated calcium channel. Nat Commun, 2021
  73. A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics. Elife, 2020
  74. An optogenetic switch for the Set2 methyltransferase provides evidence for transcription-dependent and -independent dynamics of H3K36 methylation. Genome Res, 2020
  75. Optogenetic activation of heterotrimeric G-proteins by LOV2GIVe, a rationally engineered modular protein. Elife, 2020
  76. Optogenetic Control Reveals Differential Promoter Interpretation of Transcription Factor Nuclear Translocation Dynamics. Cell Syst, 2020
  77. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
  78. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
  79. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
  80. Optogenetic Downregulation of Protein Levels to Control Programmed Cell Death in Mammalian Cells with a Dual Blue-Light Switch. Methods Mol Biol, 2020
  81. Optogenetic Control of Nucleocytoplasmic Protein Transport. Methods Mol Biol, 2020
  82. Light-Inducible CRISPR Labeling. Methods Mol Biol, 2020
  83. Long-Range Optogenetic Control of Axon Guidance Overcomes Developmental Boundaries and Defects. Dev Cell, 2020
  84. Twist-dependent ratchet functioning downstream from Dorsal revealed using a light-inducible degron. Genes Dev, 2020
  85. Combining optogenetics with sensitive FRET imaging to monitor local microtubule manipulations. Sci Rep, 2020
  86. The histone H4 basic patch regulates SAGA-mediated H2B deubiquitylation and histone acetylation. J Biol Chem, 2020
  87. Optogenetic Control for Investigating Subcellular Localization of Fyn Kinase Activity in Single Live Cells. J Mol Biol, 2020
  88. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
  89. Engineering light-controllable CAR T cells for cancer immunotherapy. Sci Adv, 2020
  90. Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation. Photochem Photobiol Sci, 2020
  91. Minimally disruptive optical control of protein tyrosine phosphatase 1B. Nat Commun, 2020
  92. Optogenetic regulation of endogenous proteins. Nat Commun, 2020
  93. Golgi-associated microtubules are fast cargo tracks and required for persistent cell migration. EMBO Rep, 2020
  94. Optogenetic control of spine-head JNK reveals a role in dendritic spine regression. eNeuro, 2020
  95. Mapping the proximity interaction network of the Rho-family GTPases reveals signalling pathways and regulatory mechanisms. Nat Cell Biol, 2019
  96. Directed evolution improves the catalytic efficiency of TEV protease. Nat Methods, 2019
  97. Visualization of a blue light transmission area in living animals using light-induced nuclear translocation of fluorescent proteins. Biochem Biophys Res Commun, 2019
  98. Locally Activating TrkB Receptor Generates Actin Waves and Specifies Axonal Fate. Cell Chem Biol, 2019
  99. Chemokine Receptor CCR7 Triggers an Endomembrane Signaling Complex for Spatial Rac Activation. Cell Rep, 2019
  100. Repurposing protein degradation for optogenetic modulation of protein activities. ACS Synth Biol, 2019
  101. Optogenetic Repressors of Gene Expression in Yeasts Using Light-Controlled Nuclear Localization. CMBE, 2019
  102. FRET-assisted photoactivation of flavoproteins for in vivo two-photon optogenetics. Nat Methods, 2019
  103. Coordination of protrusion dynamics within and between collectively migrating border cells by myosin II. Mol Biol Cell, 2019
  104. Reversible photocontrol of oxidase activity by inserting a photosensitive domain into the oxidase. BIOB, 2019
  105. VIEW-MOD: a versatile illumination engine with a modular optical design for fluorescence microscopy. Opt Express, 2019
  106. Optical control of transcription - genetically encoded photoswitchable variants of T7 RNA polymerase. Chembiochem, 2019
  107. Engineering Optogenetic Control of Endogenous p53 Protein Levels. Appl Sci, 2019
  108. Engineering proteins for allosteric control by light or ligands. Nat Protoc, 2019
  109. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
  110. Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth. Science, 2019
  111. A yeast system for discovering optogenetic inhibitors of eukaryotic translation initiation. ACS Synth Biol, 2019
  112. Optogenetic control of cell morphogenesis on protein micropatterns. bioRxiv, 2019
  113. Optogenetic Navigation of Routes Leading to Protein Amyloidogenesis in Bacteria. J Mol Biol, 2019
  114. Photo‐ECM: A Blue Light Photoswitchable Synthetic Extracellular Matrix Protein for Reversible Control over Cell–Matrix Adhesion. Adv Biosyst, 2019
  115. Smallest near-infrared fluorescent protein evolved from cyanobacteriochrome as versatile tag for spectral multiplexing. Nat Commun, 2019
  116. Engineering Improved Photoswitches for the Control of Nucleocytoplasmic Distribution. ACS Synth Biol, 2018
  117. Integrating chemical and mechanical signals through dynamic coupling between cellular protrusions and pulsed ERK activation. Nat Commun, 2018
  118. Engineered anti-CRISPR proteins for optogenetic control of CRISPR-Cas9. Nat Methods, 2018
  119. A light-gated potassium channel for sustained neuronal inhibition. Nat Methods, 2018
  120. Optogenetic manipulation of intracellular calcium by BACCS promotes differentiation of MC3T3-E1 cells. Biochem Biophys Res Commun, 2018
  121. Cyclic Stiffness Modulation of Cell‐Laden Protein–Polymer Hydrogels in Response to User‐Specified Stimuli Including Light. Adv Biosyst, 2018
  122. Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells. Sci Rep, 2018
  123. Fam49/CYRI interacts with Rac1 and locally suppresses protrusions. Nat Cell Biol, 2018
  124. Discovering selective binders for photoswitchable proteins using phage display. ACS Synth Biol, 2018
  125. Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing. Nat Protoc, 2018
  126. A Rac1-FMNL2 signaling module affects cell-cell contact formation independent of Cdc42 and membrane protrusions. PLoS ONE, 2018
  127. Rewiring Calcium Signaling for Precise Transcriptional Reprogramming. ACS Synth Biol, 2018
  128. Near-infrared light-controlled gene expression and protein targeting in neurons and non-neuronal cells. Chembiochem, 2018
  129. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
  130. Engineering a light-activated caspase-3 for precise ablation of neurons in vivo. Proc Natl Acad Sci USA, 2017
  131. An engineered photoswitchable mammalian pyruvate kinase. FEBS J, 2017
  132. A calcium- and light-gated switch to induce gene expression in activated neurons. Nat Biotechnol, 2017
  133. A light- and calcium-gated transcription factor for imaging and manipulating activated neurons. Nat Biotechnol, 2017
  134. Rac1 switching at the right time and location is essential for Fcγ receptor-mediated phagosome formation. J Cell Sci, 2017
  135. Optical control of membrane tethering and interorganellar communication at nanoscales. Chem Sci, 2017
  136. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun, 2017
  137. Light-induced protein degradation in human-derived cells. Biochem Biophys Res Commun, 2017
  138. Near-infrared optogenetic pair for protein regulation and spectral multiplexing. Nat Chem Biol, 2017
  139. Kinetics of Endogenous CaMKII Required for Synaptic Plasticity Revealed by Optogenetic Kinase Inhibitor. Neuron, 2017
  140. Investigations of human myosin VI targeting using optogenetically controlled cargo loading. Proc Natl Acad Sci USA, 2017
  141. Engineering extrinsic disorder to control protein activity in living cells. Science, 2016
  142. Optogenetic Control of Nuclear Protein Import in Living Cells Using Light-Inducible Nuclear Localization Signals (LINuS). Curr Protoc Chem Biol, 2016
  143. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
  144. Post-translational control of protein function with light using a LOV-intein fusion protein. Mol Biosyst, 2016
  145. Optogenetic control of nuclear protein export. Nat Commun, 2016
  146. Regulation of neural gene transcription by optogenetic inhibition of the RE1-silencing transcription factor. Proc Natl Acad Sci USA, 2015
  147. Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation. Elife, 2015
  148. Photocontrolled Exposure of Pro-apoptotic Peptide Sequences in LOV Proteins Modulates Bcl-2 Family Interactions. Chembiochem, 2015
  149. Optical Control of Peroxisomal Trafficking. ACS Synth Biol, 2015
  150. Optogenetic control of endogenous Ca(2+) channels in vivo. Nat Biotechnol, 2015
  151. Labelling and optical erasure of synaptic memory traces in the motor cortex. Nature, 2015
  152. An Engineered Split Intein for Photoactivated Protein Trans-Splicing. PLoS ONE, 2015
  153. Light generation of intracellular Ca(2+) signals by a genetically encoded protein BACCS. Nat Commun, 2015
  154. Control of Protein Activity and Cell Fate Specification via Light-Mediated Nuclear Translocation. PLoS ONE, 2015
  155. Junctional actin assembly is mediated by Formin-like 2 downstream of Rac1. J Cell Biol, 2015
  156. Optogenetics. Engineering of a light-gated potassium channel. Science, 2015
  157. An optogenetic upgrade for the Tet-OFF system. Biotechnol Bioeng, 2015
  158. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
  159. Structural details of light activation of the LOV2-based photoswitch PA-Rac1. ACS Chem Biol, 2014
  160. Remote control of myosin and kinesin motors using light-activated gearshifting. Nat Nanotechnol, 2014
  161. Engineering light-inducible nuclear localization signals for precise spatiotemporal control of protein dynamics in living cells. Nat Commun, 2014
  162. Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. ACS Synth Biol, 2014
  163. Rac1-dependent lamellipodial motility in prostate cancer PC-3 cells revealed by optogenetic control of Rac1 activity. PLoS ONE, 2014
  164. Optical control of the Ca2+ concentration in a live specimen with a genetically encoded Ca2+-releasing molecular tool. ACS Chem Biol, 2014
  165. A fully genetically encoded protein architecture for optical control of peptide ligand concentration. Nat Commun, 2014
  166. General method for regulating protein stability with light. ACS Chem Biol, 2013
  167. RasGRF2 Rac-GEF activity couples NMDA receptor calcium flux to enhanced synaptic transmission. Proc Natl Acad Sci USA, 2013
  168. An optogenetic tool for the activation of endogenous diaphanous-related formins induces thickening of stress fibers without an increase in contractility. Cytoskeleton (Hoboken), 2013
  169. Nuclear actin network assembly by formins regulates the SRF coactivator MAL. Science, 2013
  170. Light activated cell migration in synthetic extracellular matrices. Biomaterials, 2012
  171. Controlling the DNA cleavage activity of light-inducible chimeric endonucleases by bidirectional photoactivation. Bioconjug Chem, 2012
  172. Designing photoswitchable peptides using the AsLOV2 domain. Chem Biol, 2012
  173. Engineering a photoactivated caspase-7 for rapid induction of apoptosis. ACS Synth Biol, 2011
  174. A synthetic photoactivated protein to generate local or global Ca(2+) signals. Chem Biol, 2011
  175. Rationally improving LOV domain-based photoswitches. Nat Methods, 2010
  176. Light-mediated activation reveals a key role for Rac in collective guidance of cell movement in vivo. Nat Cell Biol, 2010
  177. A genetically encoded photoactivatable Rac controls the motility of living cells. Nature, 2009
  178. Surface sites for engineering allosteric control in proteins. Science, 2008
  179. Light-activated DNA binding in a designed allosteric protein. Proc Natl Acad Sci USA, 2008
  180. Rac1 is essential in cocaine-induced structural plasticity of nucleus accumbens neurons. Nat Neurosci, 2012
  181. LOV2-Controlled Photoactivation of Protein Trans-Splicing. Methods Mol Biol, 2017
  182. SPARK: A Transcriptional Assay for Recording Protein-Protein Interactions in a Defined Time Window. Curr Protoc, 2021