iLID

Related original publications

1. Tissue flow acts as a guidance cue for immune cell polarization and directional migration. Nat Cell Biol, 2026
2. Tunable Chemical and Optical Modulation of ER-Plasma Membrane Contact-Site Geometry, Dynamics, and Protein Recruitment with High-Fidelity Visualization. Anal Chem, 2026
3. GCL pruning of PIP3 establishes the soma-germline boundary. J Cell Biol, 2026
4. An optogenetic smart microscopy platform reveals signaling dynamics-dependent control over collective cell migration. Cell Syst, 2026
5. Long-range mutual activation establishes Rho and Rac polarity during cell migration. Nat Cell Biol, 2026
6. The regulatory logic of a dose-dependent developmental fate decision. bioRxiv, 2026
7. Optogenetic Regulation of Localization and Function of Serotonin Transporter by Modulating Its Interaction with Soluble Guanylate Cyclase. Int J Mol Sci, 2026
8. An extracellular, optogenetic antibody platform for stimulus-gated antigen recognition and modulation of cell behavior. Cell Chem Biol, 2026
9. Optical Control of Actin Network Assembly on the Supported Lipid Bilayer. Bio Protoc, 2026
10. Engineering of genetically encoded programmable calcium channel inhibitory binders. Nat Commun, 2026
11. GTPase-activating protein DLC1 spatio-temporally regulates Rho signaling. Elife, 2026
12. Optogenetic mediated contractility enables reversible control of microglial morphology and migration in vivo. Cell Rep, 2026
13. Local RhoA activation induces anillin-independent septin recruitment in interphase cells. Mol Biol Cell, 2026
14. Actin-membrane interface stress regulates Arp2/3-branched actin density during lamellipodial protrusion. bioRxiv, 2026
15. Tunable Chemical and Optical Control of ER-Plasma Membrane Contact Site Geometry and Dynamics with High-Fidelity Visualization. bioRxiv, 2026
16. Notch Signalling Plays a Role in Patterning the Ventral Mesoderm During Early Embryogenesis in Drosophila melanogaster. Int J Mol Sci, 2026
17. Oncogenic Alterations in PI3K Signaling Emulated Optogenetically Recapitulate Some Phenotypic Changes in Mammary Epithelia. ACS Synth Biol, 2026
18. Defining RNA oligonucleotides that reverse deleterious phase transitions of RNA-binding proteins with prion-like domains. Mol Cell, 2026
19. CrisprBuildr: an open-source application for CRISPR-mediated genome engineering in Drosophila melanogaster. G3 (Bethesda), 2026
20. Investigating local negative feedback of Rac activity by mathematical models and cell-motility simulations. iScience, 2026
21. GCL pruning of PIP3 establishes the soma-germline boundary. bioRxiv, 2025
22. Closed-loop optogenetic control of cell biology enables outcome-driven microscopy. Nat Commun, 2025
23. Optogenetic control of biomolecular organization reveals distinct roles of phase separation in RTK signaling. Cell Chem Biol, 2025
24. A Modular Platform for the Optogenetic Control of Small GTPase Activity in Living Cells Reveals Long-Range RhoA Signaling. bioRxiv, 2025
25. Optogenetic control of PLC-γ1 activity polarizes cell motility. bioRxiv, 2025
26. Optogenetic actin network assembly on lipid bilayer uncovers the network density-dependent functions of actin-binding proteins. Nat Commun, 2025
27. Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing. Nat Commun, 2025
28. Ras-mediated dynamic and biphasic regulation of cell migration. Proc Natl Acad Sci U S A, 2025
29. Optogenetic and chemical genetic tools for rapid repositioning of vimentin intermediate filaments. J Cell Biol, 2025
30. Dynamin-like Proteins Combine Mechano-constriction and Membrane Remodeling to Enable Two-Step Mitochondrial Fission via a "Snap-through" Instability. J Am Chem Soc, 2025
31. RhoA activation promotes ordered membrane domain coalescence and suppresses neuronal excitability. bioRxiv, 2025
32. Inward transport of organelles drives outward migration of the spindle during C. elegans meiosis. Cell Rep, 2025
33. Dynamic and Biphasic Regulation of Cell Migration by Ras. bioRxiv, 2025
34. Optogenetic control of Corynebacterium glutamicum gene expression. Nucleic Acids Res, 2024
35. Oncogenic EML4-ALK assemblies suppress growth factor perception and modulate drug tolerance. Nat Commun, 2024
36. Light-Based Juxtacrine Signaling Between Synthetic Cells. Small Sci, 2024
37. Light-induced targeting enables proteomics on endogenous condensates. Cell, 2024
38. Light-guided actin polymerization drives directed motility in protocells. bioRxiv, 2024
39. Long range mutual activation establishes Rho and Rac polarity during cell migration. bioRxiv, 2024
40. PIP5K-Ras bistability initiates plasma membrane symmetry breaking to regulate cell polarity and migration. bioRxiv, 2024
41. Systems mapping of bidirectional endosomal transport through the crowded cell. Curr Biol, 2024
42. ERK synchronizes embryonic cleavages in Drosophila. Dev Cell, 2024
43. Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag. Nat Commun, 2024
44. Endoplasmic reticulum exit sites are segregated for secretion based on cargo size. Dev Cell, 2024
45. Phospholipase C beta 1 in the dentate gyrus gates fear memory formation through regulation of neuronal excitability. Sci Adv, 2024
46. Light-Inducible Activation of TrkA for Probing Chronic Pain in Mice. ACS Chem Biol, 2024
47. Interplay of condensation and chromatin binding underlies BRD4 targeting. MBoC, 2024
48. Focal adhesions are controlled by microtubules through local contractility regulation. EMBO J, 2024
49. Endogenous OptoRhoGEFs reveal biophysical principles of epithelial tissue furrowing. bioRxiv, 2024
50. A light-controlled phospholipase C for imaging of lipid dynamics and controlling neural plasticity. Cell Chem Biol, 2024
51. Spatiotemporal Organization of Functional Cargoes by Light-Switchable Condensation in Escherichia coli Cells. JACS Au, 2024
52. Dynamic Light-Induced Protein Patterns at Model Membranes. J Vis Exp, 2024
53. Asymmetric oligomerization state and sequence patterning can tune multiphase condensate miscibility. Nat Chem, 2024
54. Simple visualization of submicroscopic protein clusters with a phase-separation-based fluorescent reporter. Cell Syst, 2024
55. Ultralow Background Membrane Editors for Spatiotemporal Control of Phosphatidic Acid Metabolism and Signaling ACS Cent Sci, 2024
56. Rapid and reversible dissolution of biomolecular condensates using light-controlled recruitment of a solubility tag. bioRxiv, 2024
57. Spatiotemporal Optical Control of Gαq-PLCβ Interactions. ACS Synth Biol, 2023
58. Photoactivation of LOV domains with chemiluminescence. Chem Sci, 2023
59. A single-component, light-assisted uncaging switch for endoproteolytic release. Nat Chem Biol, 2023
60. Local negative feedback of Rac activity at the leading edge underlies a pilot pseudopod-like program for amoeboid cell guidance. PLoS Biol, 2023
61. CaaX-motif-adjacent residues influence G protein gamma (Gγ) prenylation under suboptimal conditions. J Biol Chem, 2023
62. ActuAtor, a Listeria-inspired molecular tool for physical manipulation of intracellular organizations through de novo actin polymerization. Cell Rep, 2023
63. Light-activated microtubule-based two-dimensional active nematic. Soft Matter, 2023
64. Dynamics of an incoherent feedforward loop drive ERK-dependent pattern formation in the early Drosophila embryo. Development, 2023
65. C-terminal sequence stability profiling in Saccharomyces cerevisiae reveals protective protein quality control pathways. J Biol Chem, 2023
66. Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor. Proc Natl Acad Sci U S A, 2023
67. Senescence-induced cellular reprogramming drives cnidarian whole-body regeneration. Cell Rep, 2023
68. Opto-RhoGEFs, an optimized optogenetic toolbox to reversibly control Rho GTPase activity on a global to subcellular scale, enabling precise control over vascular endothelial barrier strength. Elife, 2023
69. Concept and considerations of a medical device: the active noise cancelling incubator. Front Pediatr, 2023
70. Cell protrusions and contractions generate long-range membrane tension propagation. Cell, 2023
71. Actuation of single downstream nodes in growth factor network steers immune cell migration. Dev Cell, 2023
72. Engineered allostery in light-regulated LOV-Turbo enables precise spatiotemporal control of proximity labeling in living cells. Nat Methods, 2023
73. Self-Regulated and Bidirectional Communication in Synthetic Cell Communities. ACS Nano, 2023
74. A disordered tether to iLID improves photoswitchable protein patterning on model membranes. Chem Commun (Camb), 2023
75. Mechanosensitive mTORC2 independently coordinates leading and trailing edge polarity programs during neutrophil migration. Mol Biol Cell, 2023
76. Motor processivity and speed determine structure and dynamics of microtubule-motor assemblies. Elife, 2023
77. Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities. Small, 2023
78. Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem Biol, 2022
79. Integrin-based adhesion compartmentalizes ALK3 of the BMPRII to control cell adhesion and migration. J Cell Biol, 2022
80. Opto-katanin, an optogenetic tool for localized, microtubule disassembly. Curr Biol, 2022
81. Optogenetic control of apical constriction induces synthetic morphogenesis in mammalian tissues. Nat Commun, 2022
82. Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases. Nat Commun, 2022
83. A non-canonical Raf function is required for dorsal-ventral patterning during Drosophila embryogenesis. Sci Rep, 2022
84. Synthetic cells with self-activating optogenetic proteins communicate with natural cells. Nat Commun, 2022
85. Persistent cell migration emerges from a coupling between protrusion dynamics and polarized trafficking. Elife, 2022
86. Optical Sensors and Actuators for Probing Proximity-Dependent Biotinylation in Living Cells. Front Cell Neurosci, 2022
87. Compartmentalization of telomeres through DNA-scaffolded phase separation. Dev Cell, 2022
88. Substratum stiffness regulates Erk signaling dynamics through receptor-level control. Cell Rep, 2021
89. Temperature-responsive optogenetic probes of cell signaling. Nat Chem Biol, 2021
90. Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis. Nat Commun, 2021
91. An Optogenetic Toolbox for Synergistic Regulation of Protein Abundance. ACS Synth Biol, 2021
92. A synthetic gene circuit for imaging-free detection of signaling pulses. Cell Syst, 2021
93. Nano-optogenetic engineering of CAR T cells for precision immunotherapy with enhanced safety. Nat Nanotechnol, 2021
94. An active tethering mechanism controls the fate of vesicles. Nat Commun, 2021
95. Circularly permuted AsLOV2 as an optogenetic module for engineering photoswitchable peptides. Chem Commun (Camb), 2021
96. Mechanical Frustration of Phase Separation in the Cell Nucleus by Chromatin. Phys Rev Lett, 2021
97. Positive feedback between the T cell kinase Zap70 and its substrate LAT acts as a clustering-dependent signaling switch. Cell Rep, 2021
98. Cell to Cell Signaling through Light in Artificial Cell Communities: Glowing Predator Lures Prey. ACS Nano, 2021
99. mem-iLID, a fast and economic protein purification method. Biosci Rep, 2021
100. Optogenetic model reveals cell shape regulation through FAK and Fascin. J Cell Sci, 2021
101. Temporal integration of inductive cues on the way to gastrulation. Proc Natl Acad Sci U S A, 2021
102. Light-inducible deformation of mitochondria in live cells. Cell Chem Biol, 2021
103. Engineering a Blue Light Inducible SpyTag System (BLISS). J Am Chem Soc, 2021
104. Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering. Nat Chem Biol, 2021
105. Endothelial cell invasion is controlled by dactylopodia. Proc Natl Acad Sci U S A, 2021
106. Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment. ACS Synth Biol, 2021
107. T cells selectively filter oscillatory signals on the minutes timescale. Proc Natl Acad Sci U S A, 2021
108. Optogenetic control of PRC1 reveals its role in chromosome alignment on the spindle by overlap length-dependent forces. Elife, 2021
109. Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling. J Biol Chem, 2021
110. Rho1 activation recapitulates early gastrulation events in the ventral, but not dorsal, epithelium of Drosophila embryos. Elife, 2020
111. Development of light-responsive protein binding in the monobody non-immunoglobulin scaffold. Nat Commun, 2020
112. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
113. Engineering combinatorial and dynamic decoders using synthetic immediate-early genes. Commun Biol, 2020
114. Multistimuli Sensing Adhesion Unit for the Self-Positioning of Minimal Synthetic Cells. Small, 2020
115. Dynamic centriolar localization of Polo and Centrobin in early mitosis primes centrosome asymmetry. PLoS Biol, 2020
116. Heterogeneous somatostatin-expressing neuron population in mouse ventral tegmental area. Elife, 2020
117. Photo-SNAP-tag, a Light-Regulated Chemical Labeling System. ACS Chem Biol, 2020
118. LITESEC-T3SS - Light-controlled protein delivery into eukaryotic cells with high spatial and temporal resolution. Nat Commun, 2020
119. Photoactivatable Cre recombinase 3.0 for in vivo mouse applications. Nat Commun, 2020
120. Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID). J Mol Biol, 2020
121. An optimized toolbox for the optogenetic control of intracellular transport. J Cell Biol, 2020
122. Light-inducible generation of membrane curvature in live cells with engineered BAR domain proteins. ACS Synth Biol, 2020
123. A Live-Cell Screen for Altered Erk Dynamics Reveals Principles of Proliferative Control. Cell Syst, 2020
124. SRRF-stream imaging of optogenetically controlled furrow formation shows localized and coordinated endocytosis and exocytosis mediating membrane remodeling. ACS Synth Biol, 2020
125. Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua. Dev Cell, 2020
126. Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling. Nat Commun, 2020
127. Tunable light and drug induced depletion of target proteins. Nat Commun, 2020
128. Dynamic organelle distribution initiates actin-based spindle migration in mouse oocytes. Nat Commun, 2020
129. Ras acts as a molecular switch between two forms of consolidated memory in Drosophila. Proc Natl Acad Sci USA, 2020
130. Engineered BRET-Based Biologic Light Sources Enable Spatiotemporal Control over Diverse Optogenetic Systems. ACS Synth Biol, 2019
131. Optimizing photoswitchable MEK. Proc Natl Acad Sci USA, 2019
132. The importance of cell-cell interaction dynamics in bottom-up tissue engineering: Concepts of colloidal self-assembly in the fabrication of multicellular architectures. Nano Lett, 2019
133. Optogenetic activation of intracellular antibodies for direct modulation of endogenous proteins. Nat Methods, 2019
134. Light controlled cell-to-cell adhesion and chemical communication in minimal synthetic cells. Chem Commun (Camb), 2019
135. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
136. Optogenetic downregulation of protein levels with an ultrasensitive switch. ACS Synth Biol, 2019
137. Reversible induction of mitophagy by an optogenetic bimodular system. Nat Commun, 2019
138. Physical Plasma Membrane Perturbation Using Subcellular Optogenetics Drives Integrin-Activated Cell Migration. ACS Synth Biol, 2019
139. Signaling Dynamics Control Cell Fate in the Early Drosophila Embryo. Dev Cell, 2019
140. A Photoactivatable Botulinum Neurotoxin for Inducible Control of Neurotransmission. Neuron, 2019
141. Mapping Local and Global Liquid Phase Behavior in Living Cells Using Photo-Oligomerizable Seeds. Cell, 2018
142. Liquid Nuclear Condensates Mechanically Sense and Restructure the Genome. Cell, 2018
143. Light-Guided Motility of a Minimal Synthetic Cell. Nano Lett, 2018
144. Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins. Small GTPases, 2018
145. Reversible Social Self-Sorting of Colloidal Cell-Mimics with Blue Light Switchable Proteins. ACS Synth Biol, 2018
146. Membrane Flow Drives an Adhesion-Independent Amoeboid Cell Migration Mode. Dev Cell, 2018
147. Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling forces as a multi-arm ensemble. Elife, 2018
148. Optogenetic reversible knocksideways, laser ablation, and photoactivation on the mitotic spindle in human cells. Methods Cell Biol, 2018
149. Optogenetic Control of Cell Migration. Methods Mol Biol, 2018
150. Light-activated protein interaction with high spatial subcellular confinement. Proc Natl Acad Sci USA, 2018
151. Dynamic blue light-switchable protein patterns on giant unilamellar vesicles. Chem Commun (Camb), 2018
152. Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker. J Cell Biol, 2017
153. Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering. ACS Synth Biol, 2017
154. Time-gated detection of protein-protein interactions with transcriptional readout. Elife, 2017
155. Intracellular production of hydrogels and synthetic RNA granules by multivalent molecular interactions. Nat Mater, 2017
156. Vesicle Docking Is a Key Target of Local PI(4,5)P2 Metabolism in the Secretory Pathway of INS-1 Cells. Cell Rep, 2017
157. Photocontrolled reversible self-assembly of dodecamer nitrilase. Bioresour Bioprocess, 2017
158. Cells lay their own tracks: optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration. J Cell Sci, 2017
159. Temporally precise labeling and control of neuromodulatory circuits in the mammalian brain. Nat Methods, 2017
160. Evolution of a split RNA polymerase as a versatile biosensor platform. Nat Chem Biol, 2017
161. The Spatiotemporal Limits of Developmental Erk Signaling. Dev Cell, 2017
162. Tuning the Binding Affinities and Reversion Kinetics of a Light Inducible Dimer Allows Control of Transmembrane Protein Localization. Biochemistry, 2016
163. Light-induced nuclear export reveals rapid dynamics of epigenetic modifications. Nat Chem Biol, 2016
164. Subcellular optogenetic activation of Cdc42 controls local and distal signaling to drive immune cell migration. Mol Biol Cell, 2016
165. Correlating in Vitro and in Vivo Activities of Light-Inducible Dimers: A Cellular Optogenetics Guide. ACS Synth Biol, 2015
166. Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins. Proc Natl Acad Sci USA, 2014
167. Optogenetic actuator - ERK biosensor circuits identify MAPK network nodes that shape ERK dynamics. Mol Syst Biol, 2022
168. Using Optogenetics to Spatially Control Cortical Dynein Activity in Mitotic Human Cells. Methods Mol Biol, 2023
169. Application of Optogenetics to Probe the Signaling Dynamics of Cell Fate Decision-Making. Methods Mol Biol, 2023
170. Nano-optogenetic CAR-T Cell Immunotherapy. Methods Mol Biol, 2024
171. Controlling the Subcellular Localization of Signaling Proteins Using Chemically Induced Dimerization and Optogenetics. Methods Mol Biol, 2024