PhyB/PIF3 & PhyB/PIF6

Related original publications

1. Light-directed evolution of dynamic, multi-state, and computational protein functionalities. Cell, 2026
2. Opto-CD28-REACT: optogenetic co-stimulatory receptor activation on non-engineered human T cells. Front Immunol, 2025
3. Optogenetic control of transgene expression in Marchantia polymorpha. Appl Plant Sci, 2025
4. Genetically-stable engineered optogenetic gene switches modulate spatial cell morphogenesis in two- and three-dimensional tissue cultures. Nat Commun, 2024
5. OptoAssay-Light-controlled dynamic bioassay using optogenetic switches. Sci Adv, 2024
6. An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts. ACS Synth Biol, 2024
7. Engineering of an Optogenetic T Cell Receptor Compatible with Fluorescence-Based Readouts. ACS Synth Biol, 2023
8. Light-inducible T cell engagers trigger, tune, and shape the activation of primary T cells. Proc Natl Acad Sci U S A, 2023
9. A biological camera that captures and stores images directly into DNA. Nat Commun, 2023
10. Multidimensional characterization of inducible promoters and a highly light-sensitive LOV-transcription factor. Nat Commun, 2023
11. PhiReX 2.0: A Programmable and Red Light-Regulated CRISPR-dCas9 System for the Activation of Endogenous Genes in Saccharomyces cerevisiae. ACS Synth Biol, 2023
12. Opto-APC: Engineering of cells that display phytochrome B on their surface for optogenetic studies of cell-cell interactions. Front Mol Biosci, 2023
13. Optical Control of Cell Signaling with Red/Far-Red Light-Responsive Optogenetic Tools in Caenorhabditis elegans. ACS Synth Biol, 2023
14. Orthogonal Light-Dependent Membrane Adhesion Induces Social Self-Sorting and Member-Specific DNA Communication in Synthetic Cell Communities. Small, 2023
15. Integration of light and temperature sensing by liquid-liquid phase separation of phytochrome B. Mol Cell, 2022
16. Spatiotemporally confined red light-controlled gene delivery at single-cell resolution using adeno-associated viral vectors. Sci Adv, 2021
17. Cross-TCR Antagonism Revealed by Optogenetically Tuning the Half-Life of the TCR Ligand Binding. Int J Mol Sci, 2021
18. Photo-dependent membrane-less organelles formed from plant phyB and PIF6 proteins in mammalian cells. Int J Biol Macromol, 2021
19. Building a Simple and Versatile Illumination System for Optogenetic Experiments. J Vis Exp, 2021
20. Liquid-liquid phase separation of light-inducible transcription factors increases transcription activation in mammalian cells and mice. Sci Adv, 2021
21. Efficient photoactivatable Dre recombinase for cell type-specific spatiotemporal control of genome engineering in the mouse. Proc Natl Acad Sci U S A, 2020
22. Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics. ACS Chem Biol, 2020
23. Multichromatic Control of Signaling Pathways in Mammalian Cells. Adv Biosyst, 2020
24. Optogenetic control of protein binding using light-switchable nanobodies. Nat Commun, 2020
25. Optogenetic control of heterologous metabolism in E. coli. ACS Synth Biol, 2020
26. CreLite: An Optogenetically Controlled Cre/loxP System Using Red Light. Dev Dyn, 2020
27. Optogenetic Rescue of a Patterning Mutant. Curr Biol, 2020
28. Optogenetic control of gene expression in plants in the presence of ambient white light. Nat Methods, 2020
29. PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants. iScience, 2020
30. Optogenetic Tuning of Ligand Binding to The Human T cell Receptor Using The opto-ligand-TCR System. Bio Protoc, 2020
31. Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics. Bio Protoc, 2020
32. Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors. Commun Biol, 2019
33. Red/Far-Red Light Switchable Cargo Attachment and Release in Bacteria-Driven Microswimmers. Adv Healthc Mater, 2019
34. Production of Phytochromes by High-Cell-Density E. coli Fermentation. ACS Synth Biol, 2019
35. High-throughput multicolor optogenetics in microwell plates. Nat Protoc, 2019
36. Optogenetic control shows that kinetic proofreading regulates the activity of the T cell receptor. Elife, 2019
37. Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome. Front Immunol, 2019
38. Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos. Methods Mol Biol, 2019
39. Optogenetic control of integrin-matrix interaction. Commun Biol, 2019
40. A size-invariant bud-duration timer enables robustness in yeast cell size control. PLoS ONE, 2018
41. Light-Induced Printing of Protein Structures on Membranes in Vitro. Nano Lett, 2018
42. Cancer mutations and targeted drugs can disrupt dynamic signal encoding by the Ras-Erk pathway. Science, 2018
43. Generic and reversible opto-trapping of biomolecules. Acta Biomater, 2018
44. Four Key Steps Control Glycolytic Flux in Mammalian Cells. Cell Syst, 2018
45. Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B. Photochem Photobiol, 2018
46. Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling. Proc Natl Acad Sci USA, 2017
47. Tracing Information Flow from Erk to Target Gene Induction Reveals Mechanisms of Dynamic and Combinatorial Control. Mol Cell, 2017
48. PhiReX: a programmable and red light-regulated protein expression switch for yeast. Nucleic Acids Res, 2017
49. Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System. Methods Mol Biol, 2017
50. A module for Rac temporal signal integration revealed with optogenetics. J Cell Biol, 2017
51. A Phytochrome-Derived Photoswitch for Intracellular Transport. ACS Synth Biol, 2017
52. An open-hardware platform for optogenetics and photobiology. Sci Rep, 2016
53. Optical manipulation of the alpha subunits of heterotrimeric G proteins using photoswitchable dimerization systems. Sci Rep, 2016
54. A bacterial phytochrome-based optogenetic system controllable with near-infrared light. Nat Methods, 2016
55. Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression. Methods Mol Biol, 2016
56. Reversible Optogenetic Control of Subcellular Protein Localization in a Live Vertebrate Embryo. Dev Cell, 2016
57. Signalling to the nucleus under the control of light and small molecules. Mol Biosyst, 2015
58. Light-Activated Nuclear Translocation of Adeno-Associated Virus Nanoparticles Using Phytochrome B for Enhanced, Tunable, and Spatially Programmable Gene Delivery. ACS Nano, 2015
59. Iterative experiment design guides the characterization of a light-inducible gene expression circuit. Proc Natl Acad Sci USA, 2015
60. Probing Yeast Polarity with Acute, Reversible, Optogenetic Inhibition of Protein Function. ACS Synth Biol, 2015
61. Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish. ACS Synth Biol, 2015
62. Benchmarking of optical dimerizer systems. ACS Synth Biol, 2014
63. Orthogonal optogenetic triple-gene control in Mammalian cells. ACS Synth Biol, 2014
64. Control of gene expression using a red- and far-red light-responsive bi-stable toggle switch. Nat Protoc, 2014
65. A red light-controlled synthetic gene expression switch for plant systems. Mol Biosyst, 2014
66. Using optogenetics to interrogate the dynamic control of signal transmission by the Ras/Erk module. Cell, 2013
67. Synthesis of phycocyanobilin in mammalian cells. Chem Commun (Camb), 2013
68. A light-inducible organelle-targeting system for dynamically activating and inactivating signaling in budding yeast. Mol Biol Cell, 2013
69. Multi-chromatic control of mammalian gene expression and signaling. Nucleic Acids Res, 2013
70. A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells. Nucleic Acids Res, 2013
71. Light-mediated control of DNA transcription in yeast. Methods, 2012
72. In silico feedback for in vivo regulation of a gene expression circuit. Nat Biotechnol, 2011
73. Light control of plasma membrane recruitment using the Phy-PIF system. Meth Enzymol, 2011
74. Spatiotemporal control of cell signalling using a light-switchable protein interaction. Nature, 2009
75. Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway. Proc Natl Acad Sci USA, 2008
76. A light-switchable gene promoter system. Nat Biotechnol, 2002
77. Engineered Cas9 extracellular vesicles as a novel gene editing tool. J Extracell Vesicles, 2022
78. Spatially Defined Gene Delivery into Native Cells with the Red Light-Controlled OptoAAV Technology. Curr Protoc, 2022