Optogenetic approaches to investigate spatiotemporal signaling during development.
blue
cyan
near-infrared
red
UV
Cryptochromes
Fluorescent proteins
LOV domains
Phytochromes
UV receptors
Review
Abstract:
Embryogenesis is coordinated by signaling pathways that pattern the developing organism. Many aspects of this process are not fully understood, including how signaling molecules spread through embryonic tissues, how signaling amplitude and dynamics are decoded, and how multiple signaling pathways cooperate to pattern the body plan. Optogenetic approaches can be used to address these questions by providing precise experimental control over a variety of biological processes. Here, we review how these strategies have provided new insights into developmental signaling and discuss how they could contribute to future investigations.
