A single-component optogenetic toolkit for reversible visualization and programmable control of microtubule dynamics.
blue
AsLOV2
CRY2/CIB1
CRY2/CRY2
A549
C. elegans in vivo
C2C12
Cos-7
H9c2
HEK293
HeLa
MEF-1
MIA PaCa-2
Neuro-2a
NIH/3T3
SK-MEL-28
U-87 MG
Control of cytoskeleton / cell motility / cell shape
Benchmarking
Abstract:
Microtubules form dynamic cytoskeletal scaffolds essential for intracellular transport, organelle positioning, and spatial organization of signaling. Their architecture and function are continuously remodeled through the concerted actions of microtubule-associated proteins (MAPs), post-translational modifications (PTMs), and molecular motors. To precisely interrogate these processes in living systems, we developed a genetically encoded, single-component optogenetic platform for spatiotemporal control of microtubule organization and dynamics. By harnessing light-induced oligomerization to regulate microtubule association, this system supports reversible microtubule labeling and plus-end tracking, localized control of tubulin PTMs, optically regulated kinesin-driven cargo transport, and inducible microtubule severing within a unified design strategy. Using these tools, we reveal how local microtubule integrity governs lysosomal trafficking and endoplasmic reticulum (ER)-associated signaling dynamics. Collectively, this modular optogenetic toolkit bridges molecular design with cytoskeletal function, offering a versatile platform to dissect how dynamic cytoskeletal architectures coordinate intracellular organization, transport, and signaling.
