Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

Qr: author:"Saki Hasegawa"
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CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.

blue CRY2/CIB1 Magnets HEK293T HeLa human fetal fibroblasts human IPSCs Cell differentiation Endogenous gene expression
Nat Methods, 11 Sep 2017 DOI: 10.1038/nmeth.4430 Link to full text
Abstract: Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
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