CRISPR-Cas9-based photoactivatable transcription systems to induce neuronal differentiation.
blue
CRY2/CIB1
Magnets
HEK293T
HeLa
human fetal fibroblasts
human IPSCs
Cell differentiation
Endogenous gene expression
Abstract:
Our improved CRISPR-Cas9-based photoactivatable transcription systems, CPTS2.0 and Split-CPTS2.0, enable high blue-light-inducible activation of endogenous target genes in various human cell lines. We achieved reversible activation of target genes with CPTS2.0 and induced neuronal differentiation in induced pluripotent stem cells (iPSCs) by upregulating NEUROD1 with Split-CPTS2.0.
