Fast manipulation of cellular cAMP level by light in vivo.
blue
euPAC
D. melanogaster in vivo
HEK293
Xenopus oocytes
Immediate control of second messengers
Neuronal activity control
Abstract:
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
