Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

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1.

Optogenetics Methods and Protocols

blue green red AsLOV2 CcaS/CcaR Cph1 CRY2/CIB1 CRY2olig DrBphP iLID LOVTRAP Magnets PAL PhyB/PIF6 TtCBD TULIP VVD YtvA 3T3-L1 B. subtilis Cos-7 E. coli H9c2 HaCaT HEK293T HeLa HFF-1 Jurkat MDA-MB-231 MKN28 mouse in vivo primary mouse T cells S. cerevisiae Schneider 2 U-2 OS Y. enterocolitica zebrafish in vivo
Methods Mol Biol, 26 Dec 2024 DOI: 10.1007/978-1-0716-4047-0 Link to full text
Abstract: This volume explores the latest advancements in the field of optogenetics and how it uses cellular light-sensing components and genetic engineering to control proteins and biological processes. The book chapters are organized into four parts. Part One focuses on intracellular optogenetic components for control of specific cell functions; Part Two looks at externally supplied light regulators that do not require genetic manipulation of target cells; Part Three highlights optogenetic control of organelles, and Part Four introduces technical tools required for light induction in optogenetic experiments, as well as a method for performing and analyzing optogenetic cell-cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Optogenetics: Methods and Protocols is a valuable resource to help researchers understand and apply the concepts of optogenetics and the underlying bioengineering principles, and establish the required technical light-illumination setups for administering light inputs and analysis of experimental outcomes.
2.

Lights on and action! Controlling microbial gene expression by light.

blue green near-infrared red BLUF domains Cryptochromes Fluorescent proteins LOV domains Phytochromes Review
Appl Microbiol Biotechnol, 20 Feb 2011 DOI: 10.1007/s00253-011-3141-6 Link to full text
Abstract: Light-mediated control of gene expression and thus of any protein function and metabolic process in living microbes is a rapidly developing field of research in the areas of functional genomics, systems biology, and biotechnology. The unique physical properties of the environmental factor light allow for an independent photocontrol of various microbial processes in a noninvasive and spatiotemporal fashion. This mini review describes recently developed strategies to generate photo-sensitive expression systems in bacteria and yeast. Naturally occurring and artificial photoswitches consisting of light-sensitive input domains derived from different photoreceptors and regulatory output domains are presented and individual properties of light-controlled expression systems are discussed.
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