Recent advances in the use of genetically encodable optical tools to elicit and monitor signaling events.
blue
cyan
red
UV
violet
BLUF domains
Cryptochromes
Fluorescent proteins
LOV domains
Phytochromes
UV receptors
Review
Abstract:
Cells rely on a complex network of spatiotemporally regulated signaling activities to effectively transduce information from extracellular cues to intracellular machinery. To probe this activity architecture, researchers have developed an extensive molecular tool kit of fluorescent biosensors and optogenetic actuators capable of monitoring and manipulating various signaling activities with high spatiotemporal precision. The goal of this review is to provide readers with an overview of basic concepts and recent advances in the development and application of genetically encodable biosensors and optogenetic tools for understanding signaling activity.
