Optogenetics Methods and Protocols
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AsLOV2
CcaS/CcaR
Cph1
CRY2/CIB1
CRY2olig
DrBphP
iLID
LOVTRAP
Magnets
PAL
PhyB/PIF6
TtCBD
TULIP
VVD
YtvA
3T3-L1
B. subtilis
Cos-7
E. coli
H9c2
HaCaT
HEK293T
HeLa
HFF-1
Jurkat
MDA-MB-231
MKN28
mouse in vivo
primary mouse T cells
S. cerevisiae
Schneider 2
U-2 OS
zebrafish in vivo
Abstract:
This volume explores the latest advancements in the field of optogenetics and how it uses cellular light-sensing components and genetic engineering to control proteins and biological processes. The book chapters are organized into four parts. Part One focuses on intracellular optogenetic components for control of specific cell functions; Part Two looks at externally supplied light regulators that do not require genetic manipulation of target cells; Part Three highlights optogenetic control of organelles, and Part Four introduces technical tools required for light induction in optogenetic experiments, as well as a method for performing and analyzing optogenetic cell-cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Optogenetics: Methods and Protocols is a valuable resource to help researchers understand and apply the concepts of optogenetics and the underlying bioengineering principles, and establish the required technical light-illumination setups for administering light inputs and analysis of experimental outcomes.
