Curated Optogenetic Publication Database

Search precisely and efficiently by using the advantage of the hand-assigned publication tags that allow you to search for papers involving a specific trait, e.g. a particular optogenetic switch or a host organism.

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1.

Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering.

blue near-infrared BphP1/Q-PAS1 FKF1/GI iLID Magnets HEK293T NIH/3T3 primary mouse cortical neurons Organelle manipulation
ACS Synth Biol, 4 Dec 2017 DOI: 10.1021/acssynbio.7b00248 Link to full text
Abstract: The organelle interface emerges as a dynamic platform for a variety of biological responses. However, their study has been limited by the lack of tools to manipulate their occurrence in live cells spatiotemporally. Here, we report the development of a genetically encoded light-inducible tethering (LIT) system allowing the induction of contacts between endoplasmic reticulum (ER) and mitochondria, taking advantage of a pair of light-dependent heterodimerization called an iLID system. We demonstrate that the iLID-based LIT approach enables control of ER-mitochondria tethering with high spatiotemporal precision in various cell types including primary neurons, which will facilitate the functional study of ER-mitochondrial contacts.
2.

Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1.

blue CRY2/CIB1 FKF1/GI HEK293T human primary dermal fibroblasts isolated MEFs NIH/3T3 Transgene expression
Nucleic Acids Res, 10 Oct 2017 DOI: 10.1093/nar/gkx804 Link to full text
Abstract: Light-inducible systems allow spatiotemporal control of a variety of biological activities. Here, we report newly optimized optogenetic tools to induce transcription with light in mammalian cells, using the Arabidopsis photoreceptor Flavin Kelch-repeat F-box 1 (FKF1) and its binding partner GIGANTEA (GI) as well as CRY2/CIB1. By combining the mutagenesis of FKF1 with the optimization of a split FKF1/GI dimerized Gal4-VP16 transcriptional system, we identified constructs enabling significantly improved light-triggered transcriptional induction. In addition, we have improved the CRY2/CIB1-based light-inducible transcription with split construct optimization. The improvements regarding the FKF1/GI- and CRY2/CIB1-based systems will be widely applicable for the light-dependent control of transcription in mammalian cells.
3.

Optogenetics: Switching with red and blue.

blue near-infrared red LOV domains Phytochromes Review
Nat Chem Biol, 17 May 2017 DOI: 10.1038/nchembio.2387 Link to full text
Abstract: Abstract not available.
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