Qr: journal:"Methods"
Showing 101 - 103 of 103 results
101.
Rationally improving LOV domain-based photoswitches.
Abstract:
Genetically encoded protein photosensors are promising tools for engineering optical control of cellular behavior; we are only beginning to understand how to couple these light detectors to effectors of choice. Here we report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of Avena sativa phototropin 1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.
102.
Activation of protein splicing with light in yeast.
Abstract:
Spatiotemporal regulation of protein function is a key feature of living systems; experimental tools that provide such control are of great utility. Here we report a genetically encoded system for controlling a post-translational process, protein splicing, with light. Studies in Saccharomyces cerevisiae demonstrate that fusion of a photodimerization system from Arabidopsis thaliana to an artificially split intein permits rapid activation of protein splicing to yield a new protein product.
103.
Fast manipulation of cellular cAMP level by light in vivo.
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Schröder-Lang, S
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Schwarzel, M
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Seifert, R
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Strünker, T
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Kateriya, S
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Looser, J
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Watanabe, M
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Kaupp, UB
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Hegemann, P
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Nagel, G
Abstract:
The flagellate Euglena gracilis contains a photoactivated adenylyl cyclase (PAC), consisting of the flavoproteins PACalpha and PACbeta. Here we report functional expression of PACs in Xenopus laevis oocytes, HEK293 cells and in Drosophila melanogaster, where neuronal expression yields light-induced changes in behavior. The activity of PACs is strongly and reversibly enhanced by blue light, providing a powerful tool for light-induced manipulation of cAMP in animal cells.
